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Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells

Overview of attention for article published in Oncotarget, August 2016
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  • High Attention Score compared to outputs of the same age and source (86th percentile)

Mentioned by

twitter
4 tweeters
patent
1 patent

Citations

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178 Dimensions

Readers on

mendeley
138 Mendeley
Title
Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells
Published in
Oncotarget, August 2016
DOI 10.18632/oncotarget.11743
Pubmed ID
Authors

Chuanzhao Zhang, Wanqing Iris Zhi, Haiquan Lu, Debangshu Samanta, Ivan Chen, Edward Gabrielson, Gregg L. Semenza

Abstract

Exposure of breast cancer cells to hypoxia increases the percentage of breast cancer stem cells (BCSCs), which are required for tumor initiation and metastasis, and this response is dependent on the activity of hypoxia-inducible factors (HIFs). We previously reported that exposure of breast cancer cells to hypoxia induces the ALKBH5-mediated demethylation of N6-methyladenosine (m6A) in NANOG mRNA leading to increased expression of NANOG, which is a pluripotency factor that promotes BCSC specification. Here we report that exposure of breast cancer cells to hypoxia also induces ZNF217-dependent inhibition of m6A methylation of mRNAs encoding NANOG and KLF4, which is another pluripotency factor that mediates BCSC specification. Although hypoxia induced the BCSC phenotype in all breast-cancer cell lines analyzed, it did so through variable induction of pluripotency factors and ALKBH5 or ZNF217. However, in every breast cancer line, the hypoxic induction of pluripotency factor and ALKBH5 or ZNF217 expression was HIF-dependent. Immunohistochemistry revealed that expression of HIF-1α and ALKBH5 was concordant in all human breast cancer biopsies analyzed. ALKBH5 knockdown in MDA-MB-231 breast cancer cells significantly decreased metastasis from breast to lungs in immunodeficient mice. Thus, HIFs stimulate pluripotency factor expression and BCSC specification by negative regulation of RNA methylation.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 138 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Finland 1 <1%
Unknown 137 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 37 27%
Researcher 19 14%
Student > Bachelor 17 12%
Student > Doctoral Student 11 8%
Student > Master 11 8%
Other 13 9%
Unknown 30 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 45 33%
Agricultural and Biological Sciences 24 17%
Medicine and Dentistry 19 14%
Pharmacology, Toxicology and Pharmaceutical Science 3 2%
Immunology and Microbiology 3 2%
Other 8 6%
Unknown 36 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 June 2021.
All research outputs
#5,705,723
of 21,360,625 outputs
Outputs from Oncotarget
#2,300
of 14,106 outputs
Outputs of similar age
#83,158
of 286,910 outputs
Outputs of similar age from Oncotarget
#148
of 1,137 outputs
Altmetric has tracked 21,360,625 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 14,106 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 286,910 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 1,137 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.