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Article Metrics

Expansion of CTCs from early stage lung cancer patients using a microfluidic co-culture model

Overview of attention for article published in Oncotarget, December 2014
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Mentioned by

news
5 news outlets
blogs
1 blog
twitter
1 tweeter
googleplus
2 Google+ users

Citations

dimensions_citation
113 Dimensions

Readers on

mendeley
139 Mendeley
Title
Expansion of CTCs from early stage lung cancer patients using a microfluidic co-culture model
Published in
Oncotarget, December 2014
DOI 10.18632/oncotarget.2592
Pubmed ID
Authors

Zhuo Zhang, Hiroe Shiratsuchi, Jules Lin, Guoan Chen, Rishindra M. Reddy, Ebrahim Azizi, Shamileh Fouladdel, Andrew C. Chang, Lin, Hui Jiang, Meghna Waghray, Gary Luker, Diane M. Simeone, Max S. Wicha, David G. Beer, Nithya Ramnath, Sunitha Nagrath

Abstract

The potential utility of circulating tumor cells (CTCs) to guide clinical care in oncology patients has gained momentum with emerging micro- and nanotechnologies. Establishing the role of CTCs in tumor progression and metastasis depends both on enumeration and on obtaining sufficient numbers of CTCs for downstream assays. The numbers of CTCs are few in early stages of cancer, limiting detailed molecular characterization. Recent attempts in the literature to culture CTCs isolated from metastatic patients using monoculture have had limited success rates of less than 20%. Herein, we have developed a novel in-situ capture and culture methodology for ex-vivo expansion of CTCs using a three dimensional co-culture model, simulating a tumor microenvironment to support tumor development. We have successfully expanded CTCs isolated from 14 of 19 early stage lung cancer patients. Expanded lung CTCs carried mutations of the TP53 gene identical to those observed in the matched primary tumors. Next-generation sequencing further revealed additional matched mutations between primary tumor and CTCs of cancer-related genes. This strategy sets the stage to further characterize the biology of CTCs derived from patients with early lung cancers, thereby leading to a better understanding of these putative drivers of metastasis.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 139 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 1%
Malaysia 1 <1%
Italy 1 <1%
France 1 <1%
Unknown 134 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 37 27%
Researcher 29 21%
Student > Master 12 9%
Student > Bachelor 11 8%
Student > Doctoral Student 10 7%
Other 25 18%
Unknown 15 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 22%
Engineering 22 16%
Biochemistry, Genetics and Molecular Biology 21 15%
Medicine and Dentistry 19 14%
Chemistry 7 5%
Other 17 12%
Unknown 22 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 53. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2014.
All research outputs
#388,919
of 15,033,985 outputs
Outputs from Oncotarget
#157
of 12,685 outputs
Outputs of similar age
#8,091
of 302,447 outputs
Outputs of similar age from Oncotarget
#2
of 233 outputs
Altmetric has tracked 15,033,985 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 12,685 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 302,447 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 233 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.