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Oncotarget

Expansion of CTCs from early stage lung cancer patients using a microfluidic co-culture model

Overview of attention for article published in Oncotarget, December 2014
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Mentioned by

news
5 news outlets
blogs
1 blog
twitter
1 X user
patent
1 patent
googleplus
2 Google+ users

Citations

dimensions_citation
169 Dimensions

Readers on

mendeley
175 Mendeley
Title
Expansion of CTCs from early stage lung cancer patients using a microfluidic co-culture model
Published in
Oncotarget, December 2014
DOI 10.18632/oncotarget.2592
Pubmed ID
Authors

Zhuo Zhang, Hiroe Shiratsuchi, Jules Lin, Guoan Chen, Rishindra M. Reddy, Ebrahim Azizi, Shamileh Fouladdel, Andrew C. Chang, Lin Lin, Hui Jiang, Meghna Waghray, Gary Luker, Diane M. Simeone, Max S. Wicha, David G. Beer, Nithya Ramnath, Sunitha Nagrath

Abstract

The potential utility of circulating tumor cells (CTCs) to guide clinical care in oncology patients has gained momentum with emerging micro- and nanotechnologies. Establishing the role of CTCs in tumor progression and metastasis depends both on enumeration and on obtaining sufficient numbers of CTCs for downstream assays. The numbers of CTCs are few in early stages of cancer, limiting detailed molecular characterization. Recent attempts in the literature to culture CTCs isolated from metastatic patients using monoculture have had limited success rates of less than 20%. Herein, we have developed a novel in-situ capture and culture methodology for ex-vivo expansion of CTCs using a three dimensional co-culture model, simulating a tumor microenvironment to support tumor development. We have successfully expanded CTCs isolated from 14 of 19 early stage lung cancer patients. Expanded lung CTCs carried mutations of the TP53 gene identical to those observed in the matched primary tumors. Next-generation sequencing further revealed additional matched mutations between primary tumor and CTCs of cancer-related genes. This strategy sets the stage to further characterize the biology of CTCs derived from patients with early lung cancers, thereby leading to a better understanding of these putative drivers of metastasis.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 175 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Malaysia 1 <1%
United States 1 <1%
Italy 1 <1%
France 1 <1%
Unknown 171 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 42 24%
Researcher 31 18%
Student > Bachelor 14 8%
Student > Master 13 7%
Student > Doctoral Student 12 7%
Other 32 18%
Unknown 31 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 19%
Biochemistry, Genetics and Molecular Biology 27 15%
Medicine and Dentistry 23 13%
Engineering 23 13%
Chemistry 8 5%
Other 19 11%
Unknown 42 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 50. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 July 2020.
All research outputs
#813,294
of 25,006,193 outputs
Outputs from Oncotarget
#324
of 14,240 outputs
Outputs of similar age
#10,396
of 373,588 outputs
Outputs of similar age from Oncotarget
#2
of 296 outputs
Altmetric has tracked 25,006,193 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 14,240 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 373,588 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 296 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.