| Title |
CRISPR/Cas9-mediated gene knockout of NANOG and NANOGP8 decreases the malignant potential of prostate cancer cells
|
|---|---|
| Published in |
Oncotarget, June 2015
|
| DOI | 10.18632/oncotarget.4293 |
| Pubmed ID | |
| Authors |
Norihiko Kawamura, Keisuke Nimura, Hiromichi Nagano, Sohei Yamaguchi, Norio Nonomura, Yasufumi Kaneda |
| Abstract |
NANOG expression in prostate cancer is highly correlated with cancer stem cell characteristics and resistance to androgen deprivation. However, it is not clear whether NANOG or its pseudogenes contribute to the malignant potential of cancer. We established NANOG- and NANOGP8-knockout DU145 prostate cancer cell lines using the CRISPR/Cas9 system. Knockouts of NANOG and NANOGP8 significantly attenuated malignant potential, including sphere formation, anchorage-independent growth, migration capability, and drug resistance, compared to parental DU145 cells. NANOG and NANOGP8 knockout did not inhibit in vitro cell proliferation, but in vivo tumorigenic potential decreased significantly. These phenotypes were recovered in NANOG- and NANOGP8-rescued cell lines. These results indicate that NANOG and NANOGP8 proteins are expressed in prostate cancer cell lines, and NANOG and NANOGP8 equally contribute to the high malignant potential of prostate cancer. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 150 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 2 | 1% |
| Peru | 1 | <1% |
| Unknown | 147 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 27 | 18% |
| Student > Ph. D. Student | 26 | 17% |
| Student > Master | 25 | 17% |
| Researcher | 18 | 12% |
| Student > Doctoral Student | 8 | 5% |
| Other | 14 | 9% |
| Unknown | 32 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 52 | 35% |
| Agricultural and Biological Sciences | 23 | 15% |
| Medicine and Dentistry | 8 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 8 | 5% |
| Chemistry | 4 | 3% |
| Other | 16 | 11% |
| Unknown | 39 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 June 2015.
All research outputs
#20,280,315
of 22,813,792 outputs
Outputs from Oncotarget
#10,571
of 14,323 outputs
Outputs of similar age
#220,328
of 264,250 outputs
Outputs of similar age from Oncotarget
#409
of 579 outputs
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