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CRISPR/Cas9-mediated gene knockout of NANOG and NANOGP8 decreases the malignant potential of prostate cancer cells

Overview of attention for article published in Oncotarget, June 2015
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1 tweeter

Citations

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Readers on

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130 Mendeley
Title
CRISPR/Cas9-mediated gene knockout of NANOG and NANOGP8 decreases the malignant potential of prostate cancer cells
Published in
Oncotarget, June 2015
DOI 10.18632/oncotarget.4293
Pubmed ID
Authors

Norihiko Kawamura, Keisuke Nimura, Hiromichi Nagano, Sohei Yamaguchi, Norio Nonomura, Yasufumi Kaneda

Abstract

NANOG expression in prostate cancer is highly correlated with cancer stem cell characteristics and resistance to androgen deprivation. However, it is not clear whether NANOG or its pseudogenes contribute to the malignant potential of cancer. We established NANOG- and NANOGP8-knockout DU145 prostate cancer cell lines using the CRISPR/Cas9 system. Knockouts of NANOG and NANOGP8 significantly attenuated malignant potential, including sphere formation, anchorage-independent growth, migration capability, and drug resistance, compared to parental DU145 cells. NANOG and NANOGP8 knockout did not inhibit in vitro cell proliferation, but in vivo tumorigenic potential decreased significantly. These phenotypes were recovered in NANOG- and NANOGP8-rescued cell lines. These results indicate that NANOG and NANOGP8 proteins are expressed in prostate cancer cell lines, and NANOG and NANOGP8 equally contribute to the high malignant potential of prostate cancer.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 130 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 2 2%
Peru 1 <1%
Unknown 127 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 26 20%
Student > Master 24 18%
Student > Ph. D. Student 23 18%
Researcher 16 12%
Student > Doctoral Student 7 5%
Other 11 8%
Unknown 23 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 50 38%
Agricultural and Biological Sciences 24 18%
Medicine and Dentistry 7 5%
Pharmacology, Toxicology and Pharmaceutical Science 7 5%
Chemistry 2 2%
Other 11 8%
Unknown 29 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 June 2015.
All research outputs
#12,602,270
of 14,259,883 outputs
Outputs from Oncotarget
#8,411
of 12,484 outputs
Outputs of similar age
#191,189
of 232,747 outputs
Outputs of similar age from Oncotarget
#313
of 463 outputs
Altmetric has tracked 14,259,883 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 12,484 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 463 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.