↓ Skip to main content

Oncotarget

Article Metrics

Exosomes from bulk and stem cells from human prostate cancer have a differential microRNA content that contributes cooperatively over local and pre-metastatic niche

Overview of attention for article published in Oncotarget, December 2015
Altmetric Badge

About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Good Attention Score compared to outputs of the same age and source (70th percentile)

Mentioned by

twitter
2 tweeters
f1000
1 research highlight platform

Citations

dimensions_citation
129 Dimensions

Readers on

mendeley
136 Mendeley
Title
Exosomes from bulk and stem cells from human prostate cancer have a differential microRNA content that contributes cooperatively over local and pre-metastatic niche
Published in
Oncotarget, December 2015
DOI 10.18632/oncotarget.6540
Pubmed ID
Authors

Catherine A. Sánchez, Eliana I. Andahur, Rodrigo Valenzuela, Enrique A. Castellón, Juan A. Fullá, Christian G. Ramos, Juan C. Triviño

Abstract

The different prostate cancer (PCa) cell populations (bulk and cancer stem cells, CSCs) release exosomes that contain miRNAs that could modify the local or premetastatic niche. The analysis of the differential expression of miRNAs in exosomes allows evaluating the differential biological effect of both populations on the niche, and the identification of potential biomarkers and therapeutic targets. Five PCa primary cell cultures were established to originate bulk and CSCs cultures. From them, exosomes were purified by precipitation for miRNAs extraction to perform a comparative profile of miRNAs by next generation sequencing in an Illumina platform. 1839 miRNAs were identified in the exosomes. Of these 990 were known miRNAs, from which only 19 were significantly differentially expressed: 6 were overexpressed in CSCs and 13 in bulk cells exosomes. miR-100-5p and miR-21-5p were the most abundant miRNAs. Bioinformatics analysis indicated that differentially expressed miRNAs are highly related with PCa carcinogenesis, fibroblast proliferation, differentiation and migration, and angiogenesis. Besides, miRNAs from bulk cells affects osteoblast differentiation. Later, their effect was evaluated in normal prostate fibroblasts (WPMY-1) where transfection with miR-100-5p, miR-21-5p and miR-139-5p increased the expression of metalloproteinases (MMPs) -2, -9 and -13 and RANKL and fibroblast migration. The higher effect was achieved with miR21 transfection. As conclusion, miRNAs have a differential pattern between PCa bulk and CSCs exosomes that act collaboratively in PCa progression and metastasis. The most abundant miRNAs in PCa exosomes are interesting potential biomarkers and therapeutic targets.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 136 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 <1%
Denmark 1 <1%
Unknown 134 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 37 27%
Student > Bachelor 18 13%
Researcher 15 11%
Student > Master 14 10%
Student > Doctoral Student 9 7%
Other 19 14%
Unknown 24 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 46 34%
Medicine and Dentistry 26 19%
Agricultural and Biological Sciences 20 15%
Neuroscience 3 2%
Chemistry 2 1%
Other 10 7%
Unknown 29 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 July 2018.
All research outputs
#10,450,981
of 17,780,770 outputs
Outputs from Oncotarget
#4,102
of 13,536 outputs
Outputs of similar age
#170,540
of 374,867 outputs
Outputs of similar age from Oncotarget
#200
of 721 outputs
Altmetric has tracked 17,780,770 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,536 research outputs from this source. They receive a mean Attention Score of 4.5. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 374,867 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 721 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.