↓ Skip to main content

Oncotarget

Article Metrics

Inhibition of O-GlcNAc transferase activity reprograms prostate cancer cell metabolism

Overview of attention for article published in Oncotarget, January 2016
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (82nd percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

blogs
1 blog
twitter
2 tweeters

Citations

dimensions_citation
42 Dimensions

Readers on

mendeley
61 Mendeley
Title
Inhibition of O-GlcNAc transferase activity reprograms prostate cancer cell metabolism
Published in
Oncotarget, January 2016
DOI 10.18632/oncotarget.7039
Pubmed ID
Authors

Harri M. Itkonen, Saurabh S. Gorad, Damien Y. Duveau, Sara E.S. Martin, Anna Barkovskaya, Tone F. Bathen, Siver A. Moestue, Ian G. Mills

Abstract

Metabolic networks are highly connected and complex, but a single enzyme, O-GlcNAc transferase (OGT) can sense the availability of metabolites and also modify target proteins. We show that inhibition of OGT activity inhibits the proliferation of prostate cancer cells, leads to sustained loss of c-MYC and suppresses the expression of CDK1, elevated expression of which predicts prostate cancer recurrence (p=0.00179). Metabolic profiling revealed decreased glucose consumption and lactate production after OGT inhibition. This decreased glycolytic activity specifically sensitized prostate cancer cells, but not cells representing normal prostate epithelium, to inhibitors of oxidative phosphorylation (rotenone and metformin). Intra-cellular alanine was depleted upon OGT inhibitor treatment. OGT inhibitor increased the expression and activity of alanine aminotransferase (GPT2), an enzyme that can be targeted with a clinically approved drug, cycloserine. Simultaneous inhibition of OGT and GPT2 inhibited cell viability and growth rate, and additionally activated a cell death response. These combinatorial effects were predominantly seen in prostate cancer cells, but not in a cell-line derived from normal prostate epithelium. Combinatorial treatments were confirmed with two inhibitors against both OGT and GPT2. Taken together, here we report the reprogramming of energy metabolism upon inhibition of OGT activity, and identify synergistically lethal combinations that are prostate cancer cell specific.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 61 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 18%
Student > Ph. D. Student 10 16%
Student > Master 9 15%
Student > Bachelor 8 13%
Student > Doctoral Student 5 8%
Other 8 13%
Unknown 10 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 19 31%
Agricultural and Biological Sciences 11 18%
Medicine and Dentistry 11 18%
Chemistry 4 7%
Pharmacology, Toxicology and Pharmaceutical Science 2 3%
Other 4 7%
Unknown 10 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 November 2016.
All research outputs
#1,242,196
of 8,760,218 outputs
Outputs from Oncotarget
#522
of 9,636 outputs
Outputs of similar age
#58,592
of 337,522 outputs
Outputs of similar age from Oncotarget
#27
of 746 outputs
Altmetric has tracked 8,760,218 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,636 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 337,522 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 746 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.